Ouch, what a title.
I had some wonderful news yesterday. Remember that I forgot to put my tubes extracted DNA in the freezer on Friday. After a very tense night, I sent an email to Supervisor first thing on Saturday explaining what had happened, and it turns out that he knew someone who would be in the lab. She, the hero, found my tubes and froze them.
So, we got all of the tubes out yesterday morning to measure them. The thing is, we don't actually know that there's DNA in there at the end of the process: it's just a drop of clear liquid. We use a clever little machine called a Qubit to see if any DNA is actually present, and estimate its concentration. We ran all of the samples through. Here are the results (samples of the same letter are from ducks of the same species from the same location):
|
Sample
|
Preparator
|
Left out overnight
|
DNA (ng/µl)
|
|
A1
|
Me
|
N
|
-
|
|
A2
|
Me
|
Y
|
-
|
|
A3
|
Me
|
Y
|
-
|
|
A4
|
Me
|
Y
|
-
|
|
A5
|
Me
|
Y
|
-
|
|
B1
|
Me
|
Y
|
-
|
|
C1
|
S
|
N
|
22.00
|
|
C2
|
S
|
N
|
4.77
|
|
C3
|
S
|
N
|
5.52
|
|
C4
|
S
|
N
|
16.20
|
|
C5
|
S
|
N
|
3.49
|
|
C6
|
S
|
N
|
4.98
|
|
C7
|
Me
|
N
|
6.90
|
|
C8
|
Me
|
N
|
5.58
|
|
C9
|
Me
|
N
|
-
|
|
C10
|
Me
|
N
|
10.40
|
|
D1
|
Me
|
Y
|
-
|
|
D2
|
Me
|
Y
|
-
|
|
D3
|
Me
|
Y
|
-
|
Only 3/13 of my samples had any DNA in them. Most of mine
were left out, which could have degraded any DNA into uselessness, and all of
those left out failed. Unfortunately, of the five I did the day before, under
supervision and that definitely did go in the freezer, two failed. And they were from different types of duck. So it looks like I need more practice at this!
I ran two more samples yesterday. We shall see how they come out this morning...
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